Research Protocol on Comparative Assessment of Tissue Regeneration by Ayurveda Medication (Amalaki Patra Mashi Ointment) and Silver Sulfadiazine in Dagdha Vrana (Burn Wound)

Background: Skin is the largest organ in the body and performs a wide variety of different function. It play an important role in the injury of the skin. Burns (Dagdha Vrana) are significant health challenge and healing can result in scar formation. Within the herbal medicine tradition the concept of Tonification and Trophorestoration is well established. Concepts of regeneration and Trophorestoration in Ayurveda provide another dimension to the area of regenerative medicine. Acharya Charaka suggests to use drugs of Amalaki to prevent Vrana. Amalaki and Silver Sulfadiazine (SSD) are the two comparative drugs chosen to evaluate the Trophorestoration concept through Dagdha Vrana in Albino rabbits.

Materials and Methods: After identification and phytochemical study of test drugs 18 adult Albino rabbits will be divided into 3 groups with six Albino Rabbits in each group. The burn wound will be induced by using metal disc, three groups have been taken in which first is control group, second is standard control, third is experimental group. Before applying experimental drugs to respective group the tissue sample will be taken from each of albino rabbits and from one of the healthy tissue sample of normal skin will be taken by Punch Biopsy for histopathalogical assay. Examination of wound area, swelling, redness, oozing will be observed. Macroscopic and Microscopic assessment of wound will be done on day 0th, 4th, 8th, and 15th, 21st day after burn. Simultaneously on 0th day and 21st day SOD and MDA test will be done. The remaining animals will be returned to the animal house for their reuse.

Expected Results: Whether Amalaki Patra Mashi ointment has more effect on troporestoration property of Twacha in Dagdha Vrana (Burn wound) than Silver Sulfadiazine.