Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria

Ahmed, J S and Lawal, S M and Fatihu, M Y and Moses, D G and Barde, I J and Kumbish, P R and Oladele, S B and Ahmed, J S and Lawal, S M and Fatihu, M Y and Moses, D G and Barde, I J and Kumbish, P R and Oladele, S B (2015) Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria. African Journal of Cellular Pathology, 4 (6). pp. 70-73. ISSN 2449-0776

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Abstract

Aim: The present study was undertaken to determine the seroprevalence of mycoplasma infection and possible isolation from local chickens in Niger State, Nigeria. We have looked into this problem using a combined MG/MS ELISA kit and cultural isolation.

Methods: A total of 552 blood samples were randomly collected from exanguinated chickens for serology. Tracheal swabs were collected into screwed cap bijou bottles containing 2ml of mycoplasma broth medium.

Results: The sero prevalence of indigenous chickens from Niger State was 91.83% by MG/MS Elisa. A total of 126 swabs yielded to the growth of avian mycoplasma on mycoplasma agar.

Item Type: Article
Subjects: Opene Prints > Medical Science
Depositing User: Managing Editor
Date Deposited: 13 Dec 2022 10:13
Last Modified: 01 Jul 2024 06:23
URI: http://geographical.go2journals.com/id/eprint/438

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