Comparative Study for Bioethanol Production from Waste Paper Using E. coli and S. cerevisiae Specie

Biomass energy is a sustainable energy source derived from plant and animal material. Bio-ethanol, bio-methanol, and biodiesel are examples of biomass fuels. One of the most significant alternative energy sources that replaces fossil fuels is bio-ethanol. The goal of this study is to create bio-ethanol from waste workplace paper. It was then subjected to weak acid hydrolysis. For each row materials such as sugarcane, A4 paper, fruit peel and hard paper after those diluted acid hydrolysis process this analysis of study validate that (99.8,99.1and 96.3) mg/g glucose content extracted from sugarcane, A4 paper, fruit peel and hard paper respectively with 1:85 (g/ml) of solid-liquid ratio. those extracted glucose content were also subjected to two different fermentation process by using E. coli and S. centeriease microbial strain followed by distillation for further processing the result analyzed by design expert software file version 13.0.5.0, study type response surface, design type Box-Bohnken and design model quadratics by three factors and levels with one response factors(alcohol content in %) then 17 runs were conducted for each microbial strain of experiments then the analysis attested or significant quantity of bio-ethanol was detected that 80.9% alcohol content obtained from 120 hr, 36°c, 5 and 36 hr, 36°c, 5 time temperature and PH value respectively by using E. coli and with other scenario by using S. centriease 97% of alcohol content obtained by 36hr, 47°c and 6.5 time temperature and PH value respectively. Therefore obesely bioethanol is cost benefit energy source so any body should be apply in the field and home level users too.