Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2

Yang, Jun and Barua, Nilakshi and Rahman, Md. Nannur and Lo, Norman and Tsang, Tsz Fung and Yang, Xiao and Chan, Paul K. S. and Zhang, Li and Ip, Margaret and Tsai, Yu-Hsuan (2021) Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2. PLOS ONE, 16 (12). e0261778. ISSN 1932-6203

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Abstract

Many CRISPR/Cas platforms have been established for the detection of SARS-CoV-2. But the detection platform of the variants of SARS-CoV-2 is scarce because its specificity is very challenging to achieve for those with only one or a few nucleotide(s) differences. Here, we report for the first time that chimeric crRNA could be critical in enhancing the specificity of CRISPR-Cas12a detecting of N501Y, which is shared by Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2 without compromising its sensitivity. This strategy could also be applied to detect other SARS-CoV-2 variants that differ only one or a few nucleotide(s) differences.

Item Type: Article
Subjects: Opene Prints > Biological Science
Depositing User: Managing Editor
Date Deposited: 01 Mar 2023 05:44
Last Modified: 01 Jul 2024 06:23
URI: http://geographical.go2journals.com/id/eprint/359

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