Identification and Characterization of Water Borne Pathogen

Aims: To carry out isolation and characterization of bacteria from water samples.

Study Design: Collection of water samples from different water sources, isolation of bacteria and establishment of pure culture, identification of bacteria

Place and Duration of Study: Sample collected from Hingotiya and Palai village of Tonk district, Rajasthan July-August, 2018.

Methodology: For initial screening of bacterial population, standard methods namely serial dilution and streaking methods were used, for identification, colony morphology and molecular characterization (16s rRNA gene) was done.

Results: The bacteriological analysis revealed that Enterobacter cloacae was present in the collected tap-water.

Conclusion: Presence of E. cloacae in tap water necessitates recommendation of frequent sanitation, treatment, management of water sources, and routine regular bacteriological assessment of all water sources for consumption as a drinking water.