Studies on Lactobacillus plantarum KFY04 Prevents Obesity in Mice through the PPAR Pathway and Alleviates Oxidative Damage and Inflammation

In this study, lactic acid bacterium, Lactobacillus plantarum KFY04, was isolated from Xinjiang yogurt,
and it was used to intervene in obese mice maintained on a 45% fat diet, and we compared its effects
to those of a commercial strain, LDSB, and L-carnitine. The results showed that the LP-KFY04
intervention mice gained weight more slowly and had lower liver, epididymal adipose, and perirenal
adipose tissue indices when compared to the other high-fat groups. Moreover, the LP-KFY04 can
reduce the formation of fat vacuoles in the liver, while also reducing adipocyte differentiation and
volume, and LP-KFY04 groups had the lowest liver and serum AST, ALT, TG, and TC levels and
lowest serum LDL-C and highest HDL-C levels among the groups maintained on a high-fat diet. LPKFY04
was also shown to mitigate obesity-associated oxidative damage and inflammatory responses.
Additionally, quantitative real-time PCR and western blot analysis examining liver and adipose tissue
expression of PPAR-α, CYP7A1, CPT1, and LPL showed an increased expression in the LP-KFY04
groups while decreased expression levels of PPAR-γ and C/EBPα relative to the other high-fat diet
groups. These results show that of the different interventions, LP-KFY04 was the most effective at
mitigating the effects of obesity than LDSB and L-carnitine. The results confirmed that LP-KEY04 has
better anti-obesity, anti-oxidative, and anti-inflammatory effects than current fermentation strains. It
indicates LP-KFY04 is a fermentation strain with potential practical value and high functionality, and it
shows that a fermentation strain should not only have good fermentation performance, but, more
importantly, it must provide more functionality on the basis of fermentation. The findings presented in
this study show that LP-KFY04 can be used to mitigate high-fat-diet-induced obesity in mice. LPKFY04
was shown to effectively reduce liver damage, fat accumulation, and oxidative and
inflammatory damage caused by obesity. It also showed a better effect than current commercial
fermented bacteria. It proves that fermentation strain LP-KFY04 not only has good fermentation
performance but also has a good functional effect.