Isolation and Molecular Characterization of BCSP-31 Gene of Brucella abortus, a Significant Tool in Making Marker and DNA Vaccines from Cases of Bovine Abortion in an Organised Dairy Farm in India

Singh, Chhabil and Kumar, Surender and Sagi, S. B. R. and Thakur, Vikas and Kumar, Amit (2023) Isolation and Molecular Characterization of BCSP-31 Gene of Brucella abortus, a Significant Tool in Making Marker and DNA Vaccines from Cases of Bovine Abortion in an Organised Dairy Farm in India. In: Newest Updates in Agriculture and Veterinary Science Vol. 4. B P International, pp. 62-70. ISBN 978-81-19102-86-0

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Abstract

Brucellosis, characterized by abortion in females and orchitis in males along with infertility in both sexes, is an important zoonotic disease in many developing countries. Isolation of Brucella organism is considered as the gold standard diagnostic method for brucellosis since it is specific and allows biotyping of the isolate, which is relevant for control of brucellosis through vaccination. This chapter reports the isolation, identification and molecular detection of Brucella abortus. Brucella abortus was detected in four clinical samples by culture isolation and was subsequently confirmed by PCR. Molecular characterization of Brucella abortus BCSP-31 gene (protein which frequently elicits an immune response during infection and a significant tool in making marker and DNA vaccines) was successfully done in this chapter.

Item Type: Book Section
Subjects: Opene Prints > Agricultural and Food Science
Depositing User: Managing Editor
Date Deposited: 07 Oct 2023 09:39
Last Modified: 07 Oct 2023 09:39
URI: http://geographical.go2journals.com/id/eprint/2540

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