In vitro Prospecting and characterization of antifungal extracted from some plants

Five extracting solvents (water, methanol, ethanol, acetonitrile and ethyl acetate) were involved to Evaluate antimicrobial of 13 different plants including different plant parts against 12 fungal species. The results showed promising antifungal activity of the ethanolic extract of C. verum and S. aromaticum that exhibited the strongest effect against P. marneffie, C. albicans, and A. niger. Two active compounds were purified using silica gel column chromatography followed by thin layer chromatography (TLC), and the characterization of active compounds was carried out by using IR, NMR and Mass spectroscopic analysis followed by determination of the minimum inhibitory concentration (MIC). First compound (A) belongs to ethanolic extract of C. verum was identified as (raffinose), The second compound (B) belong to ethanolic extract of S. aromaticum was identified as (oleanolic acid). Additionally cytotoxicity of both purified compounds was carried out, where compound (B) showed high inhibitory activity against all tested carcinoma cells, while compound (A) did not produce any antitumor activity .Also An antioxidant test was done for both compounds and the results showed that both compounds (A and B) have antioxidant activity. In conclusion, compound (B) had antifungal, antitumor and antioxidant activity while compound (A) had antifungal and antioxidant activity but, it had not antitumor activity against all tested carcinoma cells.