Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples

Shoman, Sahar and Tabl, Ashraf and Ghanem, Hussam and Nabil, Mohamed (2010) Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples. Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2 (1). pp. 1-8. ISSN 2090-0880

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Official URL: https://doi.org/10.21608/eajbsg.2010.16711

Abstract

PCR has been commonly used for genomic viral diagnosis for its sensitivity and accuracy. It showed a higher sensitivity when compared to virus isolation in tissue culture and also in antigenemia detection. Definitely, DNA samples are critical factor in PCR validity. Out of 84 serum samples subjected to this study and extracted by Wizard® DNA purification mini kit, 27 samples (32.1%) were positive PCR. While, 15 samples (17.9%) only out of the same population study (84) were positive PCR for HCMV DNA in native serum samples (without DNA extraction). This result was confirmed the importance of DNA extraction from serum samples for detection of HCMV which, subsequently lead to more sensitive diagnostic tool of an ongoing HCMV infection.

Item Type:	Article
Subjects:	Opene Prints > Biological Science
Depositing User:	Managing Editor
Date Deposited:	11 Jul 2023 03:54
Last Modified:	29 Sep 2023 12:55
URI:	http://geographical.go2journals.com/id/eprint/2341

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