In vitro Propagation and Analysis of Genetic Stability of In vitro Propagated Plants of Jaspi- A Clonal Rootstock of Prunus

A reproducible protocol for in vitro propagation of ‘Jaspi’- a Prunus rootstock was established. Jaspi is an improved rootstock dwarfing in nature and drought tolerant. The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium fortified with 0.75 mg/l Benzyl Adenine (BA) and 3 mg/l Gibberellic acid (GA3). After four weeks, the shoot buds were fragmented and transferred to the medium of same composition for in vitro shoot multiplication. In vitro elongated shoots were successfully rooted and transferred to soil. The genetic stability of micropropagated plants was analysed by RAPD, SSR and ISSR molecular markers. The results indicated that almost no somaclonal variation was detected among the micropropagated plants.