Study on Enzyme Kinetics of RNase Present in Testes

RNase A is one of the secretary enzymes of pancreas and secreted in to the digestive tract for
digestion of RNA present in the food. In RNaseA super family RNase H2 was known to involve in
protection against chromosomal instability in yeast strains where as in male wistar rats testes by
RNaseA. Drug Metosartan was proven to cause chromosomal instability in testes. So, identification of
RNase present in testes proven to be useful as it protects testes against chromosome instability.
Column chromatography was one of the techniques used here to isolate RNase present in the testes
and enzyme kinetics was performed with column isolated enzyme to know the inhibition pattern of the
enzyme, and to find K0.5 and Vmax. So, study of inhibitors that acts on enzyme must be studied
through kinetics of enzyme. RNase A present in the testes and inhibited by drug metosartan in an
allosteric manner by acting as negative modulator of the enzyme.